2017 Volume 55Annual Issue 5AM-Abstract Pages 428
There is great demand for development of in vitro three-dimensional (3D) tissues which could be used for research in the fields such as regenerative medicine and drug discovery. Piezoelectric drop-on-demand inkjet heads are attractive devices to deposit living cells in designated patterns with high precision and very high throughput. We have previously presented a novel inkjet head specialized for on-demand ejection of live cell suspensions.In this study, we constructed multi-layered 3D tissues using a process that combines ejection of cells by our inkjet heads and construction of hydrogels. We successfully produced 2-10 layer of cells stacked to 50-300um thickness by controlling the number of layers and thickness of hydrogels. These results demonstrate our inkjet heads as a powerful tool suitable for the effective ejection of living cells for the purpose of the biofabrication of living 3-D tissues.
