Observation and analysis of behavior of bubbles-surrounded cells according to the movement of acoustic distribution

Abstract

We formed aggregates with microbubbles attached around cells, and developed a technology to transport cells in the blood stream by using ultrasound apply to immunotherapy. Though we reported displacement and sustainability of aggregates by traveling wave, in this study we have manipulated aggregates by moving acoustic distribution. The nodes of standing wave were moved within the view and aggregates were tracked by image processing. When nodes swept at 20 μm/ s , aggregate moved at a rate of 30 to 60% respect to the sweep speed. Similar measurements also applied to T-cells, confirming that the kinetics could be controlled. Compared with the method of the previous study at high sound pressure, destruction of micro bubbles can be suppressed and the controllable duration time has been improved to over 30 s. It showed that the movement of acoustic distribution enable to control the dynamics of aggregates for a long time.