Behavioral Adaptogenic Glycolipid Production in Mouse Given Microbubble Bath

Abstract

Microbubbles, which are very small air bubbles with diameters less than several tens of micrometers, have chemical and physical properties different from ordinary bubbles. It was reported that bathing in CO₂ microbubbles could cause various physiologically active effects. Globopentaosylceramide (GPC) is a behavioral adaptogenic glycolipid, which increases escape time in forced swimming test. The purpose of this study was to investigate the production of GPC in the serum of mice bathed in two types of CO₂ microbubbles with different characteristics. The microbubbles were generated using two types of microbubble generators (slit type and venturi type). In a previous study, we confirmed that the CO₂ bath with microbubbles had a higher GPC production effect than that without microbubbles. In this study, more GPC was produced by mouse when the CO₂ microbubbles were generated by the slit-type generator. Microbubbles generated by the slit type generator showed a higher electrical (ζ) -potential on the gas-water interface than those generated by the venture-type generator. The results of this study revealed that mice given a microbubble bath were affected by the ζ-potential of the microbubbles, and bubbles with a high ζ-potential effectively induced the behavioral adaptogenic glycolipid.