2018 Volume Annual56 Issue Abstract Pages S179
We have developed microplate-based rapid assays for urinary biomarkers to determine urea, uric acid and creatinine, and more simple visual semi-quantitative methods such as the urine test strip. Here, a new reaction system was investigated for highly sensitive detection in the concentration range of 150 mg/L or less for microalbumin (Alb), which is a urinary protein test item recommended for early detection of kidney diseases. In this study, a reaction in which formazan showing orange or blue color was formed depending on the pH is used when the colorless water-soluble tetrazolium salt was reduced by protein under alkaline conditions. The final pH of the reaction solution was changed; in addition to quantification by absorbance at around 600 nm, the visual method was constructed. The blue green color of formazan as the final product required 10 min at 37 °C and corresponding to the Alb concentration was visible.
