Fabrication of three-dimensional tissue by perfusion culture bed

Abstract

Engineering three-dimensional tissue can give advance a cell therapy and an in vitro models for pharmaceufical research. Our laboratory has fabricated sheet-shaped cells by lowering temperature from culture surfaces grafted temperature responsive polymer and attempted to fabricate thick tissue by layering cell sheets. However, the scaling up of tissue is limited due to the lack of vessels supplying oxygen and nutrition. We report a new strategy for constructing perfusable vascularized tissues using a bioreactor having collagen-based microchannels.A co-cultured cell sheets, which contain NHDF, HUVEC were prepared. The co-cultured cell sheet was placed on the collagen-gel with microchannels perfused with the culture medium. After cultivation, microscopically observation demonstrated that endothelial cells formed tubelar structure in the co-cultured cell sheets and collagen gel. Perfusable vascularized three-dimansional tissue was constructed by cell sheet technology and the perfusion bioreactor system. These results confirmed a route to fabricate in vitro engineered an organ.