In vitro evaluation of osteoinhibition response of MC3T3-E1 on DLC coated titanium scaffolds

Abstract

For titanium and its alloy removal implant devices, adhesion with bone might cause difficulty removing the implanted devices during removal operation. We thought that Diamond Like Carbon (DLC) coated on titanium could be a material to inhibit adhesion to bone. The purpose of this study was to evaluation of osteoinhibition response of pre-osteoblast cells on the DLC coated titanium scaffolds.Commercially pure titanium grade 2 plate was coated with DLC by ionization deposition method. The MC3T3-E1 pre-osteoblast seeded on DLC coated and uncoated titanium plates for 4 days cultivation. Then, the number of cells was counted and the ALP activity was measured.The ALP activity normalized by cell number of DLC coated titanium showed a significantly lower value than the titanium. It was suggested that the DLC coated titanium devices could inhibit adhesion to bone.