2020 Volume Annual58 Issue Abstract Pages 359
Extremely high wall shear stress conditions caused by aortic stenosis (AS) have been implicated in aneurysmal dilatation and dissection of the ascending aorta. However, the detailed mechanism is still unknown. In this study, using an vascular endothelial cell (EC) - smooth muscle cell (SMC) coculture blood vessel model, we evaluated the effect of high wall shear stress on the expressions of matrix metalloproteinase (MMP) -2/-9, known to degrade aortic components. After the coculture model were exposed to fluid shear stress of 2 or 20 Pa for 24 h, MMP-2/-9 expressions of cell were assessed by Western blotting. As a result, higher expression levels of MMP-2 in ECs and MMP-9 in SMCs were detected at 20 Pa compared with 2 Pa condition. The results indicate that the elevated expression of MMP-2/-9 observed in the aortic aneurysmal lesions may be caused by high shear stress conditions.
