Magnetic nanoparticle internalization on C2C12 for construction of muscle cell sheets

抄録

In recent years, special attention has been paid to the artificial skeletal muscle. Its transplantation is expected to be useful for treatment of a variety of diseases. However, compared with living tissues, artificial skeletal muscle shows a smaller contraction force. One of the solutions for this problem is to insert magnetic nanoparticles into artificial skeletal muscle cells to support their contraction by utilizing magnetic field. Though encapsulating the magnetite in cationic liposome is previously considered necessary, the cationic liposomes possibly has an adverse effect on cells. For this purpose, we examined the amount of magnetite nanoparticles in the medium and the number of cells responding to the magnet field. Our results show that the number of cells responding to the magnet increases when the amount of magnetite increases, but it is not proportional.