Hypoallergenic spheroid production method by controlling surface properties

Abstract

It is generally known that 3D structures are required for cells to exert their function. Therefore, a method to produce large quantities of spheroids or organoids with stable size and quality is anticipated in regenerative medicine and R & D of drug using stem cells. Although various methods have been proposed for the production of spheroids or organoids, it is difficult to eliminate physical and chemical stimuli in all of these methods. The conventional methods have some problems such as a low collection rate, an expensive device, and a complicated process. We developed a new culture devise in which cells are detached from the scaffold with time, and then spheroidized even in the normal cell culture process. It is expected that spheroids can be produced easily with stable quality and the initial and operating costs are very low by using the devise.