抄録
In recent years, the number of bone marrow donors for bone marrow transplants has been increasing in advanced nations. Mainly, the Lymphocyte Citotoxicity Test (LCT) is used for the typing of HLA class I for many these volunteers. However there are two problems with LCT. (1) HLA typing with LCT requires a high technician skill. Therefore, LCT takes much money and labor, and cannot come into wide use. (2) If it is suggested that Complement do not act on some HLA immune complexes, LCT would have the disadvantage of not being adaptable for the multi type character of HLA class I. In order to realize an automatic HLA class I analyzer, we have developed an experimental equipment using following technologies. a. Auto separating leukocyte from peripheral blood. b. Positive Lymphocyte segregation in stead of using Complement. c. Positive Lymphocyte counting instead of using Microscope. d. Computer programming of HLA typing theory. Examination by an experimental equipment showed that HLA type of major sample Leukocyte went together the result of LCT. However there were some disparity of HLA type each another between the experimental equipment and LCT. These results suggest that an possibility of realizing of HLA Auto Typing Equipment will be approved, never the less above some disparity.
