The behavior of microorganisms in a methane fermenter by realtime RT-PCR

Abstract

In order to produce an efficient methane fermentation process, the behavior of microorganisms in a fermenter was examined by the methanogenic gene such as RNA.
The mcrA gene DNA amount in a fermenter was measured by realtime PCR method.
And it was evaluated for a methane generation activity.   As a result of batch experiment which changed
the CODcr load, the methane generation activity was difficult of the evaluation by the mcrA gene DNA amount per VTS amount.
On the other hand, the mcrA gene cDNA as the mcrA gene RNA amount was measured by realtime RT-PCR method with the batch experiment which changed initial pH.
The result suggested that mcrA gene cDNA per VTS amount could be evaluate more efficient of the methane generation activity.