Genotypic Analysis of Enterococci Isolated from Fecal-Polluted Water from Different Sources by Pulsed-Field Gel Electrophoresis (PFGE) for Application to Microbial Source Tracking

Takashi Furukawa; Hironori Takahashi; Terutoyo Yoshida; Yoshihiro Suzuki

doi:10.1264/jsme2.ME10174

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Genotypic Analysis of Enterococci Isolated from Fecal-Polluted Water from Different Sources by Pulsed-Field Gel Electrophoresis (PFGE) for Application to Microbial Source Tracking

Takashi Furukawa, Hironori Takahashi, Terutoyo Yoshida, Yoshihiro Suzuki

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Keywords: pulsed-field gel electrophoresis (PFGE), genotypic analysis, enterococci, fecal-polluted water, microbial source tracking

JOURNAL FREE ACCESS

2011 Volume 26 Issue 2 Pages 181-183

DOI https://doi.org/10.1264/jsme2.ME10174

View "Advance Publication" version (February 26, 2011).

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Abstract

PFGE has potential applications in the source tracking of fecal pollution in aquatic environments. We tried to distinguish the genotypes of Enterococcus faecium collected from fecal-contaminated water using PFGE. Well identified 115 strains of E. faecium were classified into 25 PFGE patterns, and characteristics distinctive to each genotype were recognized. Analysis of the characteristics of genotypes using PFGE can be used to track source of fecal pollution.

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