Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge

Yuga Hirakata; Mamoru Oshiki; Kyohei Kuroda; Masashi Hatamoto; Kengo Kubota; Takashi Yamaguchi; Hideki Harada; Nobuo Araki

doi:10.1264/jsme2.ME15154

Short Communications

Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge

Yuga Hirakata, Mamoru Oshiki, Kyohei Kuroda, Masashi Hatamoto, Kengo Kubota, Takashi Yamaguchi, Hideki Harada, Nobuo Araki

著者情報

Yuga Hirakata
Department of Civil Engineering, National Institute of Technology, Nagaoka College
Department of Science of Technology Innovation, Nagaoka University of Technology
Mamoru Oshiki
Department of Civil Engineering, National Institute of Technology, Nagaoka College
Kyohei Kuroda
Department of Environmental Systems Engineering, Nagaoka University of Technology
Masashi Hatamoto
Department of Environmental Systems Engineering, Nagaoka University of Technology
Kengo Kubota
Department of Civil and Environmental Engineering, Tohoku University
Takashi Yamaguchi
Department of Science of Technology Innovation, Nagaoka University of Technology
Hideki Harada
New Industry Creation Hatchery Center (NICHe), Tohoku University
Nobuo Araki
Department of Civil Engineering, National Institute of Technology, Nagaoka College

キーワード: Metopus ciliates, endosymbionts, Methanoregula boonei, Clostridium aminobutyricum, anaerobic granular sludge

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2015 年 30 巻 4 号 p. 335-338

DOI https://doi.org/10.1264/jsme2.ME15154

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抄録

The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates.

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