Linkage between Light Microscopic Observations and Molecular Analysis by Single-Cell PCR for Ciliates

Satoshi Shimano; Mitsuo Sanbe; Yasuhiro Kasahara

doi:10.1264/jsme2.ME08532

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Linkage between Light Microscopic Observations and Molecular Analysis by Single-Cell PCR for Ciliates

Satoshi Shimano, Mitsuo Sanbe, Yasuhiro Kasahara

著者情報

キーワード: Ciliates, single-cell PCR, 18S rRNA gene, uncultured cell, light microscopic analysis

ジャーナルフリー早期公開

論文ID: ME08532

DOI https://doi.org/10.1264/jsme2.ME08532

この記事には本公開記事があります。

The final version of this article is now available: Vol. 23 (2008) , No. 4 p.356

詳細

抄録

The molecular analysis of ciliates for identification and phylogeny is usually conducted through PCR amplification and DNA sequencing, with DNA extracted from a large number of cells. Therefore, the analysis of ciliates is limited to only those species that can be cultured. We propose a single-cell PCR procedure to overcome the difficulty in the analysis of unculturable species. The procedure has been tested on 6 Stichotrichia strains and uncultured Levicoleps biwae cells, targeting 18S rRNA gene sequences, after carrying out microscopic observations and obtaining photographic records. This procedure enables the systematic analysis of unculturable ciliate strains in natural environments by linking the morphology and genotype of a single cell.

責任著者(Corresponding author)

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