Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell

Takefumi Shimoyama; Akira Yamazawa; Yoshiyuki Ueno; Kazuya Watanabe

doi:10.1264/jsme2.ME09108

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Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell

Takefumi Shimoyama, Akira Yamazawa, Yoshiyuki Ueno, Kazuya Watanabe

著者情報

キーワード: wastewater, biomass, electricity generation, sustainable energy, microbial community

ジャーナルフリー早期公開

論文ID: ME09108

DOI https://doi.org/10.1264/jsme2.ME09108

この記事には本公開記事があります。

The final version of this article is now available: Vol. 24 (2009) , No. 2 p.188

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抄録

Quantitative and qualitative differences were analyzed between planktonic and anode-biofilm bacterial communities developed in a cassette-electrode microbial fuel cell treating starch, peptone, and fish extract. Quantitative analyses based on protein contents and rRNA-gene copy numbers indicated that planktonic microbes were over eight-times more abundant than anode-biofilm microbes. Clone-library analyses of PCR-amplified 16S rRNA gene fragments revealed the presence of bacteria affiliated with the phyla Bacteroidetes, Firmicutes, and Proteobacteria in these two communities. The most abundant sequence was affiliated with the family Porphyromonadaceae, and accounted for over 50% and 20% of all the sequences in the planktonic- and biofilm-microbe libraries, respectively.

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