Isolation of a Bacterium Possessing a Haloacid Dehalogenase from a Marine Sediment Core

Yoko Chiba; Takashi Yoshida; Norihiro Ito; Hiroshi Nishimura; Chiaki Imada; Hisato Yasuda; Yoshihiko Sako

doi:10.1264/jsme2.ME09123

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Isolation of a Bacterium Possessing a Haloacid Dehalogenase from a Marine Sediment Core

Yoko Chiba, Takashi Yoshida, Norihiro Ito, Hiroshi Nishimura, Chiaki Imada, Hisato Yasuda, Yoshihiko Sako

著者情報

キーワード: haloacid dehalogenase, monobromoacetic acid, Bacillus sp., marine sediment, biodegradation

ジャーナルフリー早期公開

論文ID: ME09123

DOI https://doi.org/10.1264/jsme2.ME09123

この記事には本公開記事があります。

The final version of this article is now available: Vol. 24 (2009) , No. 3 p.276

詳細

抄録

A monobromoacetic acid-resistant bacterium, Bacillus strain I37c, was isolated from a marine sediment core. The strain grew in a medium containing 1.8 mg mL^-1 of monobromoacetic acid. It produced constitutively a 2-haloacid dehalogenase that catalyzed the dehalogenation of monobromoacetic acid, monochloroacetic acid, and both L- and D-2-chloropropionic acid. The optimal pH and temperature for the activity measured using a partly purified enzyme were similar to those of known group I haloacid dehalogenases.

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