Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining

Takashi Baba; Naoko Inoue; Nobuyasu Yamaguchi; Masao Nasu

doi:10.1264/jsme2.ME11324

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Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining

Takashi Baba ^†, Naoko Inoue ^†, Nobuyasu Yamaguchi ^†, Masao Nasu ^†

著者情報

キーワード: Legionella pneumophila, rapid enumeration, microcolony, growth activity, freshwater

ジャーナルフリー早期公開

論文ID: ME11324

DOI https://doi.org/10.1264/jsme2.ME11324

この記事には本公開記事があります。

The final version of this article is now available: Vol. 27 (2009), No. 3 pp. 1203160361-1203160361

詳細

抄録

In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water samples collected from 30 bathing facilities, with similar sensitivity of a conventional plate-counting method. These results suggest that the microcolony method combined with fluorescent antibody staining could be useful as a monitoring technique for the prevention of Legionnaires' disease through the early detection of L. pneumophila in freshwater.

責任著者(Corresponding author)

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