Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil

Yoshinori Sato; Hiroyuki Ohta; Takao Yamagishi; Yong Guo; Tomoyasu Nishizawa; M. Habibur Rahman; Hisao Kuroda; Task Kato; Masanori Saito; Ikuo Yoshinaga; Kazuyuki Inubushi; Yuichi Suwa

doi:10.1264/jsme2.ME11330

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Detection of Anammox Activity and 16S rRNA Genes in Ravine Paddy Field Soil

Yoshinori Sato, Hiroyuki Ohta, Takao Yamagishi, Yong Guo, Tomoyasu Nishizawa, M. Habibur Rahman, Hisao Kuroda, Task Kato, Masanori Saito, Ikuo Yoshinaga, Kazuyuki Inubushi, Yuichi Suwa

著者情報

キーワード: anaerobic ammonium oxidation, ravine paddy field soil, nitrogen removal

ジャーナルフリー早期公開

論文ID: ME11330

DOI https://doi.org/10.1264/jsme2.ME11330

この記事には本公開記事があります。

The final version of this article is now available: Vol. 27 (2009), No. 3 pp. 1202170358-1202170358

詳細

抄録

An anammox assay involving a ¹⁵N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N₂ production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus ‘Brocadia fulgida’, Ca. ‘B. anammoxidans’, and Ca. ‘Kuenenia stuttgartiensis’. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.

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