Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311

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A Fine-Scale Phylogenetic Analysis of Free-Living Burkholderia Species in Sugarcane Field Soil
Kanako Tago, Hideomi Itoh, Yoshitomo Kikuchi, Tomoyuki Hori, Yuya Sato, Atsushi Nagayama, Takashi Okubo, Ronald Navarro, Tomo Aoyagi, Kentaro Hayashi, Masahito Hayatsu
著者情報
キーワード: Burkholderia, Illumina sequencing, sugarcane field
ジャーナル フリー 早期公開

論文ID: ME14122

この記事には本公開記事があります。
The final version of this article is now available: Vol. 29 (2014), No. 4 pp. 434-437
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The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (107 g−1 soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the Burkholderia community structure; however, their impacts were not significant considering the overall genus size.

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© 2014 Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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Bulletin of Japanese Society of Microbial Ecology（日本微生物生態学会報）

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