Abstract
We have developed a line-scanning multiplex coherent anti-Stokes Raman scattering (CARS) microscope for label free imaging. The developed microscope utilizes 10 femtosecond and 5 picosecond Ti:Sapphire lasers as light sources of a broad and a narrow spectral bandwidth. The spectrum of the 10 femtosecond laser expands from 746 to 836 ran, which corresponds from 600 to 2300 cm^<-1> in Raman shift when the 5 picosecond laser is set to 714 nm. CARS spectrum of arterial vessel wall of Watanabe heritable hyperlipidemic rabbit is demonstrated using the developed system. To improve the imaging speed, we have modified the system using line-scanning for parallel excitation and detection by inserting a Powel lens on excitation laser beams. The experimental results of line-scanning CARS imaging of polystyrene beads is also presented.
