Abstract
Influenza A virus is an infection source of the epidemic. Two types of protein exist on the virus surface. One is hemagglutinin (HA) and another is neuraminidase (NA). There are 16 kinds of HA and 9 kinds of NA, which makes a combination of 144 kinds of the virus subtype. Subtype H1N1 and subtype H3N2 frequently infect human every year. Some of these subtypes can be the cause of the pandemic. For sensing the various types of the virus, a new material detecting the virus which can be easily and quickly produced, is required. In this study, we focused on the heavy chain antibodies derived from camelids (VhH) from a view point of industrial applications. The size of VhH is comparatively small (15 kDa), which makes it easy to be produced. The phage library displaying VhHs was constructed and a monoclonal VhH which bound to HA of H1N1 subtype specifically was selected by bio-panning. The characteristic of the VhH was evaluated by the Enzyme-linked immuno-sorbent assay (ELISA).
