抄録
Traumatic brain injury caused by traffic accidents, blow, falls and contact sports, is an important public health problem. Diffuse Axonal Injury (DAI) shows a symptom of no consciousness without either cerebral contusion or hematoma. It is thought that DAI is occurred at the damage of the cell level within the brain widely. However, the pathogenesis of DAI is not yet fully clarified, and the effective diagnosis and cure are not established. The ultimate goal in this study is to clarify the threshold for neuronal damage DAI at the cell level of the brain. Therefore, we exposed axons in cultured neuronal cells to the impulsive strain and the effect of the impulsive strain on axonal injury was evaluated at the cell level. Neural stem cells (NSCs) were used as cultured neuronal cells. NSCs can be proliferate and differentiate into neurons and glia. When cultured in medium, NSCs extend the axons towards any direction. However, the in vivo neuronal cells of the human have a characteristic of the orientation. Therefore, the direction of axons growth is controlled by using micro grooves which matched with the size of the cell. Culture surface is used by micro grooves on polydimethylsiloxane (PDMS). It is transcribed from micro groove were generated by using photolithography on resist material (SU8) and the Si substrate. This study has investigated the effects of impulsive strain on axons in cultured neurons differentiated from NSCs.
