Abstract
The aim of this study is to develop hybrid tubular structure of PLCL porous tube and mesenchymal stem cells (MSCs). Such graft was constructed by layering MSC sheets on porous PLCL tubular graft. Cell proliferation assay confirmed that the number of cells increased steadily during 11 days of in vitro study. Moreover, it was also found that co-culture cell sheet of MSCs and endothelial cells (ECs) exhibited higher proliferation rate than that of MSCs' monoculture cell sheet, indicating that the co-culture cell sheet can modulate higher proliferation of cells. Tensile mechanical testing of the hybrid tubes was also conducted and it was found that the mechanical properties of the hybrid tube with co-culture cell sheets tended to be larger than those of the tube with monoculture sheets.
