抄録
Stem cell-based self-assembled tissues (scSATs) bio-synthesized from synovium-derived mesenchymal stem cell have a great potential for the repair and regeneration of biological soft tissues. However, the mechanical properties of the scSAT were insufficient for clinical application to a relatively large tissue defect. A candidate solution to the problem is to promote the generation of the extracellular matrix in the scSAT via a cell/tissue cultivation under cyclic strain. In the present study, MSCs were cultured in the presence of ascorbic acid for 7 days on the elastic tissue culture plates, then subsequently cultured under cyclic tensile strain for 7days, with 10 % at 1 Hz, for either 1 hour (1 h group), 3 hours (3 h group) or 5 hours (5 h group) a day. Specimens cultured for 14 days under static condition on the elastic tissue culture plate were defined as static group. Morphological observation of the surface structure of 1 h group was performed using a scanning electron microscope. The scSAT specimens of 3 h group, 5 h group and static group were subjected to tensile testing. Results revealed that the load at failure and tensile strength of the scSAT were higher in 3 h and 5 h groups than in static group. One-way analysis of variance demonstrated a significant effect of duration of strain application on the tensile strength and modulus of the scSATs. Morphological observation indicated that collagen fiber-like structure aligned parallel with the direction of strain application in 1 h group. It is suggested that the structural alignment due to the cell/tissue cultivation under cyclic strain resulted in the improvement of mechanical properties of the scSATs.
