Abstract
Stem cell-based self-assembled tissue (scSAT) biosynthesized from synovium-derived mesenchymal stem cells (MSCs) has a great potential for repairing and regenerating ligaments, tendons and cartilage. However, the mechanical and structural properties of the scSAT were insufficient for clinical applications. A candidate solution to the problem is to promote the generation of the extracellular matrix in the scSAT using a special culture on static magnetic field. A magnetic exposure system Halbach cylinder-like array was manufactured with 8 neodymium magnets. A preliminary test revealed that the magnetic exposure system generated 0.674 T magnetic field. MSC was cultured on 0.674 T static magnetic field generated the magnetic exposure system. As a result, orientation intensity, cell adhesion area and aspect ratio of MSC cultured on static magnetic field were same as MSC cultured without static magnetic field. Cell occupation area of MSC cultured on static magnetic field was higher than MSC cultured without static magnetic field.
