Abstract
Stem cell-based self-assembled tissue (scSAT) biosynthesized from synovium-derived mesenchymal stem cell (MSC) has a great potential for the repair and regeneration of cartilage. For the improvement of the mechanical properties of the scSAT it is a candidate solution to promote chondrogenic differentiation of MSCs. In the present study, cell/tissue culture substratum having various elastic moduli were developed using a polydimethylsiloxane (PDMS) with different mixing ratios of main agent and curing agent. Rabbit synovium-derived mesenchymal stem cells were cultured on the substrates. Results revealed that cell adhesion area became narrower and aspect ratio became higher as the substratum became softer. It is suggested that the differentiation of MSCs is dependent on the elasticity of culture substratum.
