抄録
In the field of regenerative medicine, it is expected to separate disease-causing cells from healthy cells by electrical method with label-free and high efficiency. The purpose of this study is to find the velocity differences between disease-causing cells and healthy cells under AC electric field in an electrodes multilayered microchannel. We prepared intracellular charge differed three cell types: wild type cells (WT), wild type cells with green fluorescent protein (GFPT) and wild type cells with green fluorescent protein histone (GFP-HT). The cell dynamic movement of each type was detected by Particle Tracking Velocimetry method. Velocity differences were found and discussed among WT, GFPT and GFP-HT around the AC voltage applied electrode. The present results provide a great significance for separation of disease-causing cells from healthy cells by histone numbers in the microchannel.
