Abstract
This paper presents a method of detecting viral RNA by using Quartz Crystal Microbalance (QCM). QCM is mass sensor for detecting biomolecules. We fabricated microfluidic chip with QCM and immobilized Peptide Nucleic Acid (PNA) on the surface of the electrode of QCM. PNA is one of the artificial nucleic acid and it can capture viral RNA specifically. Therefore, we used the gold nanoparticles conjugated anti-nucleoprotein antibody to increase sensing mass and improve sensing limit of QCM. As a result, we succeeded in detecting viral RNA (5 x 10^3 pfu).
