Abstract
A cell manipulation is important technology to develop novel medical therapy and medical applications. AC electrokinetics is useful for the cell manipulation. The applied electric field in the cell dispersion generates forces on cells with dielectrophoresis (DEP) and flows caused by the AC electrokinetics. The system of sharp tip electrode with AC electrokinetics is one of the optimal combinations to manipulate cells for biological and medical applications. In this study, hepatocytes motion and the fluid flow were investigated using the sharp tip electrode under several applied voltage conditions, applied frequency conditions, the particle size and liquid conductivity. The characteristics of the cell manipulation and the generating mechanism of the electric induced flow with AC electroosmosis were investigated.