Abstract
This study aims at forming a fluorescent complex between HAp coatings with ligands of amino acid by using CIP process in order to suppress cytotoxicity of conventional fluorescent HAp complex and also enhance antibacterial properties by visible light irradiation. Three amino acids, phenylalanine, tryptophan and tyrosine were used in the CIP process. By applying pressures from 200 MPa to 800 MPa, fluorescence of HAp with the three amino acids was successfully observed by UV irradiations. In the case of highly compressed samples, fluorescence in some regions was shifted to longer wavelength. Cytotoxicity assessments were conducted by using MC3T3-E1 osteoblast cells. Observing optical density of mediums after cultivations revealed that there were no significant differences among five groups in cases of same culture periods. The results demonstrated that biocompatibility of HAp/amino acid complex was similar to HAp itself.
