Abstract
This paper presents the creation of heterologous cell pattern by cell culture surface processing technique based on photolithography. The alginate gel was used as cell adhesion inhibition material. The alginate gel was formed on a glass plate by spin-coating. The alginate gel pattern was created by wet etching process or lift-off process along the pattern of photoresist. The cell micropattern was created along the alginate gel micropattern. The alginate gel micropattern that inhibits adhesion of cells was removed by ethylenediaminetetraacetic acid (EDTA). After removing the alginate gel pattern, new cells were disseminated to the same dish, and new cells adhered to the alginate gel removed section. Heterologous cell pattern was created by patterning of the alginate gel based on photolithography.
