Abstract
In this study, a sensor-actuator is developed to enforce stimulation and to detect change of mechanical properties of cultured mouse iPS (induced Pluripotent Stem) cells. The glass micro probe is fixed on the micro sensor-actuator. This probe is improved to direct contact with differentiated neuron cells from iPS cells. The device can apply arbitrary stimulation and measure stiffness of single cell. In order to check sensing ability of the developed device, evaluation experiment was performed with silicone rubber blocks whose Shore hardness (Hs) is known. Experimental studies have been performed in the process of the mouse iPS cells differentiating to neuron cell. In this process, mouse iPS cells are applied dynamic and static stimulation and the measurement of stiffness lasts for 7 days every 12 hours before and after stimulation. In the experiment, the neuron cells responded sensitively with dynamic and static mechanical stimulations and that the cell morphology and stiffness change depending on stimulation.
