Effect of dynamic stimulation on reconstruction of damaged neuronal cells colony

Abstract

Neural cells, which are generally said to be hard to recover from physcical damage, are equipped with function of a sensor that senses stimuli and extends axons has been elucidated. In this study, we investigated the effect of the dynamic stimulation on damaged mouse neuron colonies in neuronal network reconstruction. The neural cells differentiated from iPS cells are cultured for 5 days, and after the cells have sufficiently grown, the neuronal cells colony is damaged. Next, dynamic stimulation is applied for 6 days after the damage applied. To apply dynamic stimulation, we use a small piezo electric vibration stage. The vibration condition is set as the amplitude of 60 µm, the frequency of 5 Hz, and the interval stimulation with every 12hours in the direction perpendicular to the damaged surface. As a result, it was confirmed that the neurites are reconstructed from the damage by the applied dynamic stimulation.