Cell Suspension Culture Method Using Agitation by Ultrasound Irradiation

Abstract

In this study, we report a novel cell suspension culture method. When culturing large quantities of cells, suspension culture is generally used. We performed suspension culture using agitation by acoustic streaming. First, we manufactured the suspension culture devise that can generate acoustic streaming and agitate medium in a cell culture flask. Next, we performed cell culture experiments using the devise. As a result, the cell proliferation rate in the case of irradiating ultrasound was higher than that in the case of not irradiating ultrasound. Therefore, cell suspension culture can be more efficient using agitation by ultrasound irradiation.