Microfluidic channel for measurement of cell adhesion strength using ultrasonic

Abstract

Cell adhesion is closely related to various cellular properties. However, there is no effective method to measure cell adhesion. Meanwhile, we have developed an enzyme-free detachment method, which can detach cells by irradiating ultrasonic. Hence, we set out to develop a method for measuring the adhesive force of cells by irradiating ultrasonic from the bottom of the dish. As a preliminary experiment for this purpose, we fabricated a cell detachment microfluidic device for irradiation of ultrasonic on a cell in order to focus on observation of one cell. The microchannel was made of a cover glass and PDMS, and the ultrasonic vibrator was fabricated by using a transducer with a glass plate and a piezoelectric ceramic plate. By using this device, the mesenchymal stem cells were cultured in the microchannel, and ultrasonic irradiated to a single cell cultured in the microchannel. By increasing the output voltage of the transducer, cells detached by the ultrasonic were observed.