Effect of frequency on ultrasound-induced damage on cells in an in-vitro culture environment

Abstract

Many types of bioengineering applications have been developed in these days, which accelerates studies for effective cell culture systems. Use of ultrasound has gotten attention in the effort to realize effective cell culture since cell functions can be regulated by ultrasound. Hence, for developing the techniques based on ultrasound exposure, it is required to reveal the tolerance of cells for ultrasound exposure (ultrasound tolerance). We thus developed the cell culture system irradiating ultrasound to cultured cells, which can be used for the study of ultrasound tolerance. With the system, ultrasound with different frequencies and exposure times and Chinese hamster ovary (CHO-K1) cells were employed, and the ultrasound tolerance has been investigated. As a result, the ultrasound tolerance of CHO-K1 depends on the frequency and exposure time of the ultrasound. From this result, we argue that the developed system can be used to study the ultrasound tolerance of cultured cells, which is helpful for developing effective cell culture systems based on ultrasound techniques.