Recently, the process for high concentration saccharification has been focused in order to manufacture bio-ethanol. For short time and high concentration saccharification, the developments of an enzymatic hydrolysis process were demanded. In this study, the enzymatic hydrolysis process with a wet mill using sample of Japanese cedar wood powder and a solids-liquid separation by the filter press using the residue after the enzymatic hydrolysis process were carried out. As a result of this, the enzymatic hydrolysis process with the wet mill increased an initial saccharification speed and maximum saccharification ratio of holocellulose in case of 10% solids concentration of wood powder. And the residue and glucose solution collected by the filter-press were slightly unaffected by miniaturization of wood powder during the enzymatic hydrolysis process with the wet mill.
