Ultra-high pressure underwater shock wave was applied to treatment microbe. The imploding detonation of propane-oxygen mixture was used to generate underwater shock wave in sample holder. We investigated imploding detonation wave in approximately cone-shaped combustion chamber having maximum inner diameter of 60 mm. As microbe of the treatment experiments, Microcystis sp. and Anemia salina's eggs were used. As a result, we could completely treatment the Microcystis sp. by more than 3 times treatments of the underwater shock wave that maximum pressure was about 100 MPa. On the other hand, the experimental result using Anemia salina's eggs showed that hatchability was less than 1% at over 2 times treatments of about 100MPa under water shock wave.