Abstract
Hepatocyte transplantation is one of the cell transplantation techniques for the certain diseases of the liver.It has considerable potential as an alternative treatment of solid organ transplantation.In particular,it is indispensable treatment to control ammonia metabolism for a newborn baby who has liver dysfunctions.It is important to investigate the mechanical property of the hepatocyte while medical transactions.In this study,collagenase perfusion method was used for cell separation.Damage of the hepatocyte in shear flow were measured using trypan blue solution with a micro channel.As a result,hepatocyte injury situation was observed with optical microscope.The destruction conditions of the hepatocyte were clarified and the optimal infusion condition of the hepatocyte transplantation was estimated.
