Abstract
Leukocyte rolling is physiological phenomenon and it is important process to migrate to inflammatory sites after the leukocyte adhere to endothelial cells. It is said that leukocyte rolling closely relates to arteriosclerosis or cancer metastasis. However, the mechanism of leukocyte adhesion to endothelial cells hass not been sufficiently understood. In this study, we mimicked the leukocyte rolling in a microchannel by coating E-selectin on a epoxy-functionalized glass substrate. Uniform coating of E-selectin by FITC-conjugated anti-E-selectin was preliminary confirmed. We measured leukocyte rolling behavior on the substrate. As the result, we succeeded in the observation of the decreased leukocyte velocity by the interaction between leukocyte and adhesion molecules.
