Abstract
For the purpose of separating particles in horse blood at the size threshold of 20μm, a simple microfluidic device with the depth of 110μm contracted flow part was developed using a rapid prototyping. It was found that the particles more than 30μm were separated from all over the horse blood by 100%, but the improvement of the micro channel structure is necessary for the separation of the particle of around 20μm. And moreover, the yeast cells with the diameter of 5μm were selected as sample detection particles after the separation. The newly flow cytometry with the wavelength level of 400-650nm was developed to detect the yeast cells. It was found that the excitation fluorescence spectra patterns typically presented in yeast cells.
