Abstract
Glycocalyx layer on endothelial cells (ECs) has filtering function related with the specific mass transfer. To evaluate the mass transfer, time-course measurement of concentration distribution in the glycocalyx layer is one of the effective approaches. However, spatial resolution of conventional methods was not enough to observe the layer. In the present study, in order to clarify the detail mechanism of the mass transfer, we developed a novel investigation method of mass transfer in a glycocalyx layer of an EC using a super resolution LIF. As the result of a demonstration of the method, we discovered that the effective filtering area was about 28 times wider than the glycocalyx layer of 0.43 μm thickness.
