超音波援用ドップラーOCT（UA-OCDV）を用いた組織透水性のマイクロ断層評価

抄録

In recent years, the regenerative medicine has been being developed due to the improvement of tissue engineering, e.g. autologous regenerative tissue by three-dimensional culture. In this study, Ultrasound-assisted Doppler Velocigraphy (UA-OCDV) is constructed to measure the tissue displacement and the inerstitial fluid flow, tomographically, non-contactly and non-invasively at the micrometer resolution. This is based on Doppler OCT, which can visualize Doppler velocity inside tissue by applying phase analysis to low coherence interference signals. Furthermore, a High Intensity Focused Ultrasound (HIFU) transducer is introduced into Doppler OCT as a non-contact loading device of periodical acoustic radiation pressure. UA-OCDV was experimentally applied to flow field around scaffold. As a result, UA-OCDV was able to provide the tomographic visualization of periodical flow velocity field. Flow velocity through fibers of hydrophilic scaffold was observed to increase 140% compared to that of hydrophobic one. The nano-scale water film generated around hydrophilic surface is considered to reduce the flow resistance. In conclusion, it was therefore suggested that UA-OCDV had a potential of diagnosing tissue permeability non-contactly and non-inasively.