Abstract
Currently, chronic donor shortage is a concern. Therefore much attention has been gathered in regenerative medicine. Technology is necessary in order to achieve this, high accuracy patterning biomaterial into living cells. PELID method is to solve this problem is possible. However, there also exists a biomaterial that inhibits the formation of Taylor cone. This was thought to be due to loss of precision occurs. To solve this problem, we invented a method to insert a tungsten wire inside the nozzle.