Abstract
The method for rapid and reversible motion control of a single biomolecular motor is a key technology for future goal of integrating biomoletular motors with organic-inorganic hybrid nanosystems. A device for controlling the temperature locally under the microscope with fast response, 71.5K/s and 56.9K/s for temperature rise and fall respectively, was manufactured. Rapid and reversible angular velocity control of a single F1-ATPase, a rotary biomolecular motor, was achieved by switching the temperature. The rotation assay at the temperature higher than around 50℃ was realized thanks to the rapid switching of the temperature. The torque of F1-ATPase was found to increase at higher temperature by subtracting the effect of viscous drag of water.
