抄録
We have developed a few types of cell arraying device based on micro fabrication. One is for patch-clamp measurements, where the orifice is precisely machined using a focused ion-beam (FIB). To achieve leak-tight clamping, the irregularity of the orifice shape must be minimized, and FIB is found to be suitable for this purpose. Another is the use of lower-cost laser-machining, in combination with the surface treatment with a cell membrane-anchoring chemical, BAM, by which the membrane adheres to the orifice plate to spontaneously seal the gap between the cell and the orifice. Also developed is a cell arraying device made of organic thin-films having laser-machined orifices. In contrast to the conventional devices based on anisotropic etching of the silicon wafer, the method provides a low-cost disposable cell arrayer.
