Abstract
In this study, we have proposed and developed an in vitro embryo production device which allows three steps of sperm selection, fertilization and culture on a microdevice. To realize this concept, we integrated the sperm sorting function combining with swim-up and swim-down methods onto the device. To evaluate the device functions, sperm sorting experiment, embryo production experiment, embryo culture experiment and IVF experiment have been carried out. As results, we concluded that the device has ability to produce high quality embryos by integrating the sperm sorting function. This concept will open and enhance the management of an individual embryo for assisted reproductive technology, livestock breeding, and fundamental stage research by further development.
