抄録
Online monitoring of cellular metabolism involved in cell growth and differentiation contributes to evaluate cellular condition in cell-based assays. However, conventional methods of cellular monitoring measure discrete time-based data of cell conditions, because the methods rely on intermittent sample extraction. Therefore, a novel online measurement method is highly required to obtain more detailed and dynamic information about cell activities. In this study, we developed a microfluidic device integrated with a glucose sensor having an automatic calibration function. To validate functions of the device, we measured glucose consumption of HepG2 cells and it toxicities using the device. As a result of our preliminary test, the online measurement of the difference of glucose concentration by cells succeeded using the device.
