Abstract
There is a lack of effective therapies for serious uterus diseases which causes infertility .Such approaches as organ transplants or artificial organs still have safety and technical problems that substitutional bioartificial tissue engineering method is required to regenerate the patients' organs with gestational function. In the conventional uterus tissue engineering researches, it has been reported that seeding mesenchymal stem cells (MSCs) on the scaffold is effective for reconstruction of uterus-like tissues. However, the function of MSCs seeding remains to be seen and the fertility rate of recipients is still low. In this research, we seeded MSCs with autofluorescence of GFP(Green Fluorescent Protein) on decellularized rat uterus tissue so that we could track the behavior of MSCs after transplantation. We then investigated the the tissue 4wk after transplantation and observed a tendency of MSCs' differentiation into smooth muscle cells. It is suggested that this may be caused by tensile strain added to the recellularized tissues aimed at cell inroads into tissues.
