The Proceedings of Conference of Kanto Branch
Online ISSN : 2424-2691
ISSN-L : 2424-2691
2021.27
Session ID : 10A08
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Image reconstruction and quantification of dead yeast cell rate and cell concentration using electrical impedance measurement and convolutional neural network
Kento NISHIBAYASHI*Daisuke KAWASHIMAHiromichi OBARAMasahiro TAKEI
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Abstract

A neural network using CNN was constructed to visualize the dead cell rate and volume concentration of yeast cells, and accuracy verification was performed using test data by simulation. Regarding the construction of CNN, the normalized imaginary impedance Ψ was used as the input. When actually learning, it was observed that the model loss converged around 1000 learning times. In addition, when the dead cell rate and volume concentration were visualized using test data, it was confirmed that the accuracy of 1 or 2 cell regions was high, but that of 3 or 4 cells was low. In addition, when the accuracy is quantitatively verified, the image error shows a value as close to 0 as possible in the most accurate data, and the highest image error is IQ = 0.064 [-] for the dead cell rate and IQ = 0.012 [-] for the volume concentration. From this result, it was found that this method is useful when the number of cell regions is small, but there is room for improvement when the number of cell regions is large.

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© 2021 The Japan Society of Mechanical Engineers
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